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Deletion of the α immunoglobulin chain membrane‐anchoring region reduces but does not abolish IgA secretion
Author(s) -
Amin Rada,
Carrion Claire,
Decourt Catherine,
Pinaud Eric,
Cogné Michel
Publication year - 2012
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2012.03557.x
Subject(s) - immunoglobulin a , plasma cell , immunoglobulin class switching , biology , antibody , b cell , immunoglobulin heavy chain , breakpoint cluster region , immunoglobulin d , j chain , secretion , secretory component , immunoglobulin light chain , cellular differentiation , microbiology and biotechnology , immunology , receptor , immunoglobulin g , endocrinology , gene , genetics
Summary Class switching and plasma cell differentiation occur at a high level within all mucosa‐associated lymphoid tissues. The different classes of membrane immunoglobulin heavy chains are associated with the Ig α /Ig β heterodimer within the B‐cell receptor (BCR). Whether BCR isotypes convey specific signals adapted to the corresponding differentiation stages remains debated but IgG and IgA membranes have been suggested to promote plasma cell differentiation. We investigated the impact of blocking expression of the IgA‐class BCR through a ‘ α Δtail’ targeted mutation, deleting the C α immunoglobulin gene membrane exon. This allowed us to evaluate to what extent class switching and plasma cell differentiation can be concurrent processes, allowing some α Δtail +/+ B cells with an IgM BCR to directly differentiate into IgA plasma cells and yield serum secreted IgA in spite of the absence of membrane IgA + B lymphocytes. By contrast, in secretions the secretory IgA was very low, indicating that J‐chain‐positive plasma cells producing secretory IgA overwhelmingly differentiate from previously class‐switched membrane IgA + memory B cells. In addition, although mucosa‐associated lymphoid tissues are a major site for plasma cell accumulation, α Δtail +/+ mice showed that the gut B‐cell lineage homeostasis is not polarized toward plasma cell differentiation through a specific influence of the membrane IgA BCR.