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The stromal cell antigen CD248 (endosialin) is expressed on naive CD8 + human T cells and regulates proliferation
Author(s) -
Hardie Debbie L.,
Baldwin Mathew J.,
Naylor Amy,
Haworth Oliver J.,
Hou Tie Zheng,
Lax Sian,
Curnow S. John,
Willcox Nick,
MacFadyen John,
Isacke Clare M.,
Buckley Christopher D.
Publication year - 2011
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2011.03437.x
Subject(s) - biology , cd8 , cytotoxic t cell , stromal cell , microbiology and biotechnology , t cell , antigen , immunology , cancer research , immune system , genetics , in vitro
Summary CD248 (endosialin) is a transmembrane glycoprotein that is dynamically expressed on pericytes and fibroblasts during tissue development, tumour neovascularization and inflammation. Its role in tissue remodelling is associated with increased stromal cell proliferation and migration. We show that CD248 is also uniquely expressed by human, but not mouse (C57BL/6), CD8 + naive T cells. CD248 is found only on CD8 + CCR7 + CD11a low naive T cells and on CD8 single‐positive T cells in the thymus. Transfection of the CD248 negative T‐cell line MOLT‐4 with CD248 cDNA surprisingly reduced cell proliferation. Knock‐down of CD248 on naive CD8 T cells increased cell proliferation. These data demonstrate opposing functions for CD248 on haematopoietic (CD8 + ) versus stromal cells and suggests that CD248 helps to maintain naive CD8 + human T cells in a quiescent state.