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Nucleotide‐binding and oligomerization domain‐like receptors and retinoic acid inducible gene‐like receptors in human tonsillar T lymphocytes
Author(s) -
Petterson Terese,
Månsson Anne,
Riesbeck Kristian,
Cardell Lars O.
Publication year - 2011
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2011.03414.x
Subject(s) - nod1 , biology , cd3 , receptor , microbiology and biotechnology , nod2 , cd28 , peripheral blood mononuclear cell , immune system , t cell , immunology , cd8 , biochemistry , innate immune system , in vitro
Summary Nucleotide‐binding and oligomerization domain (NOD) ‐like receptors (NLRs) and retinoic acid‐inducible gene (RIG) ‐like receptors (RLRs) are recently discovered cytosolic pattern‐recognition receptors sensing mainly bacterial components and viral RNA, respectively. Their importance in various cells and disorders is becoming better understood, but their role in human tonsil‐derived T lymphocytes remains to be elucidated. In this study, we evaluated expression and functional relevance of NLRs and RLRs in human tonsillar CD3 + T lymphocytes. Immunohistochemistry, real‐time RT‐PCR and flow cytometry revealed expression of NOD1, NOD2, NALP1, NALP3, NAIP, IPAF, RIG‐1, MDA‐5 and LGP‐2 at mRNA and protein levels. Because of the limited number of ligands (iE‐DAP, MDP, Alum, Poly(I:C)/LyoVec), functional evaluation was restricted to NOD1, NOD2, NALP3 and RIG‐1/MDA‐5, respectively. Stimulation with the agonists alone was not enough to induce activation but upon triggering via CD3 and CD28, a profound induction of proliferation was seen in purified CD3 + T cells. However, the proliferative response was not further enhanced by the cognate ligands. Nonetheless, in tonsillar mononuclear cells iE‐DAP, MDP and Poly(I:C)/LyoVec were found to augment the CD3/CD28‐induced proliferation of tonsillar mononuclear cells. Also, iE‐DAP and MDP were found to promote secretion of interleukins 2 and 10 as well as to up‐regulate CD69. This study demonstrates for the first time a broad range of NLRs and RLRs in human tonsillar T cells and that NOD1, NOD2 and RIG‐1/MDA‐5 act synergistically with αCD3 and αCD28 to induce proliferation of human T cells. Hence, these results suggest that these receptors have a role in T‐cell activation.