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Osteopontin is up‐regulated and associated with neutrophil and macrophage infiltration in glioblastoma
Author(s) -
Atai Nadia A.,
Bansal Manju,
Lo Cheungh,
Bosman Joost,
Tigchelaar Wikky,
Bosch Klazien S.,
Jonker Ard,
De Witt Hamer Philip C.,
Troost Dirk,
McCulloch Christopher A.,
Everts Vincent,
Van Noorden Cornelis J. F.,
Sodek Jaro
Publication year - 2011
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2010.03335.x
Subject(s) - osteopontin , biology , infiltration (hvac) , cancer research , glioma , cancer , cancer cell , pathology , gene signature , extracellular , in vitro , immunology , gene , gene expression , medicine , microbiology and biotechnology , physics , genetics , thermodynamics , biochemistry
Summary Osteopontin (OPN) is a glycophosphoprotein with multiple intracellular and extracellular functions. In vitro , OPN enhances migration of mouse neutrophils and macrophages. In cancer, extracellular OPN facilitates migration of cancer cells via its RGD sequence. The present study was designed to investigate whether osteopontin is responsible for neutrophil and macrophage infiltration in human cancer and in particular in glioblastoma. We found that in vitro mouse neutrophil migration was RGD‐dependent. In silico , we found that the OPN gene was one of the 5% most highly expressed genes in 20 out of 35 cancer microarray data sets in comparison with normal tissue in at least 30% of cancer patients. In some types of cancer, such as ovarian cancer, lung cancer and melanoma, the OPN gene was one of those with the highest expression levels in at least 90% of cancer patients. In glioblastoma, the most invasive type of brain tumours/glioma, but not in lower grades of glioma it was one of the 5% highest expressed genes in 90% of patients. In situ , we found increased protein levels of OPN in human glioblastoma versus normal human brain confirming in silico results. OPN protein expression was co‐localized with neutrophils and macrophages. In conclusion, OPN in tumours not only induces migration of cancer cells but also of leucocytes.