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GATA‐binding protein‐3 regulates T helper type 2 cytokine and ifng loci through interaction with metastasis‐associated protein 2
Author(s) -
Hwang Soo Seok,
Lee Sumin,
Lee Wonyong,
Lee Gap Ryol
Publication year - 2010
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2010.03271.x
Subject(s) - chromatin , cytokine , biology , transcription factor , psychological repression , jurkat cells , microbiology and biotechnology , transfection , locus (genetics) , gata4 , gene , gene expression , t cell , genetics , immune system
Summary GATA‐binding protein‐3 (GATA‐3) regulates the T helper type 2 (Th2) cytokine locus through induction of chromatin remodelling. However, the molecular mechanism for this is poorly understood. To understand this mechanism better, we screened GATA‐3 interacting proteins using affinity purification and mass spectrometry. We found that GATA‐3 bound to metastasis‐associated protein 2 (MTA‐2), a component of the NuRD chromatin remodelling complex. GATA‐3 and MTA‐2 in turn bound to several regulatory regions of the Th2 cytokine locus and the ifng promoter. Cell transfection assay showed that MTA‐2 acted as an antagonist with GATA‐3 in the expression of Th2 cytokines, but co‐operated with GATA‐3 in the repression of the ifng gene expression. These results suggest that GATA‐3 interacts with MTA‐2 to co‐ordinately regulate Th2 cytokine and ifng loci during T helper cell differentiation.