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Salmonella pathogenicity island 1 differentially modulates bacterial entry to dendritic and non‐phagocytic cells
Author(s) -
Bueno Susan M.,
Wozniak Aniela,
Leiva Eduardo D.,
Riquelme Sebastián A.,
Carreño Leandro J.,
Hardt WolfDietrich,
Riedel Claudia A.,
Kalergis Alexis M.
Publication year - 2010
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2009.03233.x
Subject(s) - salmonella , biology , microbiology and biotechnology , salmonella enterica , effector , pathogenicity island , phagocytosis , type three secretion system , mutant , secretion , virulence , bacteria , gene , genetics , biochemistry
Summary Salmonella enterica serovar Typhimurium can enter non‐phagocytic cells, such as intestinal epithelial cells, by virtue of a Type Three Secretion System (TTSS) encoded in the Salmonella Pathogenicity Island 1 (SPI‐1), which translocates bacterial effector molecules into the host cell. Salmonella can also be taken up by dendritic cells (DCs). Although the role of SPI‐1 in non‐phagocytic cell invasion is well established, its contribution to invasion of phagocytic cells has not been evaluated. Here, we have tested the invasive capacity of a S. Typhimurium strain lacking a key component of its TTSS‐1 (ΔInvC) leading to defective translocation of SPI‐1‐encoded effectors. Whereas this mutant Salmonella strain was impaired for invasion of non‐phagocytic cells, it was taken up by DCs at a significantly higher rate than wild‐type Salmonella . Similar to wild‐type Salmonella , the ΔInvC mutant strain retained the capacity to avoid antigen presentation to T cells. However, mice infected with the ΔInvC mutant strain showed higher survival rate and reduced organ colonization. Our data suggest that, besides promoting phagocytosis by non‐phagocytic cells, SPI‐1 modulates the number of bacteria that enters DCs. The SPI‐1 could be considered not only as an inducer of epithelial cell invasion but as a controller of DC entry.