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Human peripheral γδ T cells possess regulatory potential
Author(s) -
Kühl Anja A.,
Pawlowski Ni.,
Grollich Katja,
Blessenohl Maike,
Westermann Jürgen,
Zeitz Martin,
Loddenkemper Christoph,
Hoffmann Jörg C.
Publication year - 2009
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2009.03162.x
Subject(s) - il 2 receptor , t cell , cd28 , interleukin 21 , biology , foxp3 , cytotoxic t cell , transforming growth factor beta , cytokine , microbiology and biotechnology , immunology , transforming growth factor , immune system , in vitro , biochemistry
Summary Deficiency in γδ T cells aggravates colitis in animal models suggesting that γδ T cells have regulatory properties. Therefore, proliferation, suppression and cytokine secretion of human γδ T cells were determined in vitro . Human peripheral γδ T cells were isolated from the whole blood of healthy donors by magnetic antibody cell sorting technology. The proliferation after CD3/CD28 stimulation was measured by 3 [H]thymidine incorporation. Interferon‐γ (IFN‐γ), interleukin‐2 (IL‐2), transforming growth factor‐β (TGF‐β) and IL‐10 concentrations were measured by enzyme‐linked immunosorbent assay; TGF‐β messenger RNA was also measured by reverse transcription–polymerase chain reaction. The expression of latency associated peptide (LAP), a TGF‐β complex component, intracellular cytokine content and T helper cell proliferation were measured by flow cytometry. Human γδ T cells showed poor proliferation upon CD3/CD28 stimulation and suppressed T helper cell growth stronger than CD4 + CD25 + T cells, although γδ T cells were FOXP3 negative. They secreted little IL‐2 but high concentrations of IFN‐γ, IL‐10 and TGF‐β. When looking at LAP expression the Vδ1 subset was found to be the main TGF‐β producer compared to Vδ2 T cells. Taken together, peripheral γδ T cells have in vitro a more potent regulatory potential than CD4 + CD25 + cells regarding T helper cell suppression. This is most likely the result of strong TGF‐β secretion, particularly by the Vδ1 subset.