Premium
Accelerated induction of mycobacterial antigen‐specific CD8 + T cells in the Mycobacterium tuberculosis ‐infected lung by subcutaneous vaccination with Mycobacterium bovis bacille Calmette–Guérin
Author(s) -
Begum Dilara,
Umemura Masayuki,
Yahagi Ayano,
Okamoto Yuko,
Hamada Satoru,
Oshiro Kiyotetsu,
Matsuzaki Goro
Publication year - 2009
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2009.03141.x
Subject(s) - mycobacterium bovis , cd8 , tuberculosis , mycobacterium tuberculosis , tuberculosis vaccines , vaccination , antigen , immunology , bcg vaccine , major histocompatibility complex , cytotoxic t cell , interferon gamma , virology , biology , medicine , immune system , microbiology and biotechnology , pathology , biochemistry , in vitro
Summary Both CD4 + and CD8 + T cells are important in protection against Mycobacterium tuberculosis infection. To evaluate the effect of vaccination with Mycobacterium bovis bacille Calmette–Guérin (BCG) on the CD8 + T‐cell response to pulmonary M. tuberculosis infection, we analyzed the kinetics of CD8 + T cells specific to the mycobacterial Mtb32a 309–318 epitope, which is shared by M. tuberculosis and M. bovis BCG, in the lung of mice infected with M. tuberculosis . The CD8 + T cells were detected by staining lymphocytes with pentameric major histocompatibility complex (MHC) class I H‐2D b– Mtb32a 209–318 peptide complex and were analysed by flow cytometry. Mtb32a‐specific CD8 + T cells became detectable on day 14, and reached a plateau on day 21, in the lung of M. tuberculosis ‐infected unvaccinated mice. Subcutaneous vaccination with M. bovis BCG in the footpads induced Mtb32a‐specific CD8 + T cells in the draining lymph nodes (LNs) on day 7 and their numbers further increased on day 14. When M. bovis BCG‐vaccinated mice were exposed to pulmonaryinfection with M. tuberculosis 4 weeks after vaccination, the Mtb32a‐specific CD8 + T cells in the infected lung became detectable on day 7 and reached a plateau on day 14, which was 1 week earlier than in the unvaccinated mice. The pulmonary CD8 + T cells from the BCG‐vaccinated M. tuberculosis ‐infected mice produced interferon‐γ in response to Mtb32a 209–318 peptide on day 7 of the infection, whereas those of unvaccinated mice did not. The results demonstrate that induction of mycobacterial antigen‐specific protective CD8 + T cells in the M. tuberculosis ‐infected lung is accelerated by subcutaneous vaccination with M. bovis BCG.