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Genomic expression analysis by single‐cell mRNA differential display of quiescent CD8 T cells from tumour‐infiltrating lymphocytes obtained from in vivo liver tumours
Author(s) -
Zhang Wei,
Ding Jianqing,
Qu Yan,
Hu Hongliang,
Lin Meihua,
Datta Amit,
Larson Alan,
Liu George E.,
Li Biaoru
Publication year - 2009
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2008.02926.x
Subject(s) - biology , microbiology and biotechnology , cd8 , suppression subtractive hybridization , cytotoxic t cell , cell cycle , t cell , gene silencing , cell , cancer research , gene , gene expression , immunology , genetics , cdna library , antigen , immune system , in vitro
Summary We performed a genomic study combining single‐cell mRNA differential display and RNA subtractive hybridization to elucidate CD8 T‐cell quiescence/ignorance. By comparing actively maintained quiescent CD8 T cells from liver tumour tumour‐infiltrating lymphocytes (TILs) with quiescent T cells at the single‐cell level, we identified differentially expressed candidate genes by high‐throughput screening and comparative analysis of expressed sequence tags (ESTs). While genes for the T‐cell receptor, tumour necrosis factor (TNF) receptor, TNF‐related apoptpsis inducing ligand (TRAIL) and perforin were down‐regulated, key genes such as Tob, transforming growth factor (TGF)‐β, lung Krüpple‐like factor (LKLF), Sno‐A, Ski, Myc, Ets‐2 repressor factor (ERF) and RE1‐silencing transcription factor (REST/NRSF) complex were highly expressed in the quiescent TIL CD8 cells. Real‐time polymerase chain reaction (PCR) further confirmed these results. A regulation model is proposed for actively maintained quiescence in CD8 T cells, including three components: up‐regulation of the TGF‐β pathway, a shift in the MYC web and inhibition of the cell cycle.