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Enhanced secretion of interferon‐γ by bovine γδ T cells induced by coculture with Mycobacterium bovis ‐infected dendritic cells: evidence for reciprocal activating signals
Author(s) -
Price Sally J.,
Hope Jayne C.
Publication year - 2009
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2008.02889.x
Subject(s) - secretion , interleukin 12 , biology , antigen presentation , il 2 receptor , innate immune system , antigen presenting cell , t cell , immunology , microbiology and biotechnology , major histocompatibility complex , interferon gamma , dendritic cell , immune system , antigen , cytotoxic t cell , in vitro , biochemistry
Summary Evidence suggests that γδ T cells form part of the innate immune response to Mycobacterium bovis infection. Dendritic cells (DCs) are capable of secreting high levels of interleukin‐12 (IL‐12) following infection with mycobacteria and can induce interferon‐γ (IFN‐γ) secretion by natural killer and γδ T cells We investigated the innate interactions occurring between WC1 + γδ T cells and M. bovis ‐infected DCs. Following coculture with M. bovis ‐infected DCs, secretion of IFN‐γ and expression of CD25 and major histocompatibility complex class II on WC1 + γδ T cells were significantly enhanced. Reciprocal enhancement of IL‐12 secretion by the DCs was also observed and this interaction was found to be contact dependent. We hypothesize that there is an early, transient signal between the WC1 + γδ T cells and the DCs, which promotes the synthesis of biologically active IL‐12, and which is dependent upon cell–cell contact. Reciprocal signals including IL‐12 are then delivered to WC1 + γδ cells, which leads to the enhanced secretion of IFN‐γ, and the up‐regulation of activation markers and antigen presentation molecules by the WC1 + γδ T cells. These interactions are likely to form a critical part of the T helper type 1‐conditioning response of DCs to M. bovis .