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Co‐ordinated expression of lymphoid and myeloid specific transcription factors during B‐1b cell differentiation into mononuclear phagocytes in vitro
Author(s) -
Popi Ana F.,
Motta Fabiana L. T.,
Mortara Renato A.,
Schenkman Sergio,
Lopes José D.,
Mariano Mario
Publication year - 2009
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2008.02883.x
Subject(s) - phagocyte , mononuclear phagocyte system , biology , transcription factor , myeloid , irf8 , b cell , pax5 , immunoglobulin gene , monocyte , cellular differentiation , antibody , microbiology and biotechnology , immunology , gene , phagocytosis , genetics
Summary We previously demonstrated that B‐1b cells can undergo differentiation to acquire a mononuclear phagocyte phenotype upon attachment to substrate in vitro . Here we followed the expression of surface markers and transcription factors during this differentiation. B‐1b cells spontaneously express both myeloid and lymphoid restricted transcription factors. When induced to differentiate into a phagocyte, the lymphoid genes E box protein (E2A), early B‐cell factor (EBF), paired box 5 (Pax5) are down‐modulated, while expression of genes related to myeloid commitment is sustained. Furthermore, B‐1b cell‐derived phagocytes (B‐1CDPs) decrease immunoglobulin M (IgM) expression but retain the expression of the heavy chain variable gene VH11 or VH12, an immunoglobulin gene rearrangement predominantly expressed by B‐1 cells. The maintenance of lymphoid characteristics in B‐1CDPs characterizes a unique type of phagocyte, not related to monocyte‐derived macrophages.