Premium
The modulatory effects of lipopolysaccharide‐stimulated B cells on differential T‐cell polarization
Author(s) -
Xu Hui,
Liew Lip Nyin,
Kuo I Chun,
Huang Chiung Hui,
Goh Denise LiMeng,
Chua Kaw Yan
Publication year - 2008
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2008.02832.x
Subject(s) - lipopolysaccharide , cd40 , cd86 , antigen , b cell , stimulation , t cell , naive b cell , microbiology and biotechnology , biology , immunology , chemistry , antigen presenting cell , immune system , antibody , endocrinology , cytotoxic t cell , in vitro , biochemistry
Summary Lipopolysaccharide (LPS) is a major component of environmental microbial products. Studies have defined the LPS dose as a critical determining factor in driving differential T‐cell polarization but the direct effects of LPS on individual antigen‐presenting cells is unknown. Here, we investigated the effects of LPS doses on naive B cells and the subsequent modulatory effects of these LPS‐activated B cells on T‐cell polarization. The LPS was able to induce a proliferative response starting at a dose of 100 ng/ml and was capable of enhancing antigen internalization at a dose of 1 μg/ml in naive B cells. Following LPS stimulation, up‐regulation of the surface markers CD40, CD86, I‐A d , immunoglobulin M, CD54 and interleukin‐10 production, accompanied by down‐regulation of CD5 and CD184 (CXCR4) were observed in a LPS dose‐dependent manner. Low doses (< 10 ng/ml) of LPS‐activated B cells drove T helper type 2 polarization whereas high doses (> 0·1 μg/ml) of LPS‐activated B cells resulted in T regulatory type 1 cell polarization. In conclusion, LPS‐activated B cells acquire differential modulatory effects on T‐cell polarization. Such modulatory effects of B cells are dependent on the stimulation with LPS in a dose‐dependent manner. These observations may provide one of the mechanistic explanations for the influence of environmental microbes on the development of allergic diseases.