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Expression of Langerin/CD207 reveals dendritic cell heterogeneity between inbred mouse strains
Author(s) -
Flacher Vincent,
Douillard Patrice,
AïtYahia Smina,
Stoitzner Patrizia,
ClairMoninot Valérie,
Romani Nikolaus,
Saeland Sem
Publication year - 2008
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2007.02785.x
Subject(s) - langerin , cd11c , biology , lymph , lymphatic system , immunology , cd8 , spleen , dendritic cell , phenotype , cd1 , pathology , microbiology and biotechnology , immune system , genetics , medicine , natural killer t cell , gene
Summary Langerin/CD207 is expressed by a subset of dendritic cells (DC), the epithelial Langerhans cells. However, langerin is also detected among lymphoid tissue DC. Here, we describe striking differences in langerin‐expressing cells between inbred mouse strains. While langerin + cells are observed in comparable numbers and with comparable phenotypes in the epidermis, two distinct DC subsets bear langerin in peripheral, skin‐draining lymph nodes of BALB/c mice (CD11c high CD8α high and CD11c low CD8α low ), whereas only the latter subset is present in C57BL/6 mice. The CD11c high subset is detected in mesenteric lymph nodes and spleen of BALB/c mice, but is virtually absent from C57BL/6 mice. Similar differences are observed in other mouse strains. CD11c low langerin + cells represent skin‐derived Langerhans cells, as demonstrated by their high expression of DEC‐205/CD205, maturation markers, and recruitment to skin‐draining lymph nodes upon imiquimod‐induced inflammation. It will be of interest to determine the role of lymphoid tissue‐resident compared to skin‐derived langerin + DC.