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Antigen processing and CD24 expression determine antigen presentation by splenic CD4 + and CD8 + dendritic cells
Author(s) -
Askew David,
Harding Clifford V.
Publication year - 2008
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2007.02711.x
Subject(s) - antigen presentation , cytotoxic t cell , antigen , antigen processing , antigen presenting cell , cd8 , major histocompatibility complex , biology , cross presentation , mhc class i , immunology , cd1 , t cell , dendritic cell , microbiology and biotechnology , immune system , interleukin 21 , biochemistry , in vitro
Summary To examine heterogeneity in dendritic cell (DC) antigen presentation function, murine splenic DCs were separated into CD4 + and CD8 + populations and assessed for the ability to process and present particulate antigen to CD4 + and CD8 + T cells. CD4 + and CD8 + DCs both processed exogenous particulate antigen, but CD8 + DCs were much more efficient than CD4 + DCs for both major histocompatibility complex (MHC) class II antigen presentation and MHC class I cross‐presentation. While antigen processing efficiency contributed to the superior antigen presentation function of CD8 + DCs, our studies also revealed an important contribution of CD24. CD8 + DCs were also more efficient than CD4 + DCs in inducing naïve T cells to acquire certain effector T‐cell functions, for example generation of cytotoxic CD8 + T cells and interferon (IFN)‐γ‐producing CD4 + T cells. In summary, CD8 + DCs are particularly potent antigen‐presenting cells that express critical costimulators and efficiently process exogenous antigen for presentation by both MHC class I and II molecules.