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Age‐related changes in the occurrence and characteristics of thymic CD4 + CD25 + T cells in mice
Author(s) -
Kozlowska Ewa,
Biernacka Marzena,
Ciechomska Marzena,
Drela Nadzieja
Publication year - 2007
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2007.02667.x
Subject(s) - biology , microbiology and biotechnology , chemistry , immunology
Summary Natural regulatory CD4 + CD25 + T cells play an important role in preventing autoimmunity by maintaining self‐tolerance. They express CD25 constitutively and are produced in the thymus as a functionally mature T‐cell population. Changes in the potential of these cells to regulate the activity of conventional effector lymphocytes may contribute to an increased susceptibility to infection, cancer and age‐associated autoimmune diseases. In this study we demonstrated that the thymi of aged mice are populated by a higher percentage of CD4 + CD25 + thymocytes than in young animals. The expression of several surface markers (CD69, CD5, CD28, CTLA‐4, CD122, FOXP3), usually used to characterize the phenotype of CD4 + CD25 + T regulatory cells, was compared between young and aged mice. We also examined the ability of sorted thymus‐deriving regulatory T cells of young and aged BALB/c mice to inhibit the proliferation of lymph node lymphocytes activated in vitro . Natural regulatory T cells isolated from the thymi of young mice suppress the proliferation of responder lymph node cells. We demonstrated that thymus‐deriving CD4 + CD25 + T cells of old mice maintain their potential to suppress the proliferation of activated responder lymphocytes of young mice. However, their potential to inhibit the proliferation of old responder T cells is abrogated. Differences in the occurrence and activity of CD4 + CD25 + thymocytes between young and old animals are discussed in relation to the expression of these surface markers.