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BAC to immunology – bacterial artificial chromosome‐mediated transgenesis for targeting of immune cells
Author(s) -
Sparwasser Tim,
Eberl Gérard
Publication year - 2007
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2007.02605.x
Subject(s) - bacterial artificial chromosome , transgenesis , biology , transgene , immune system , gene targeting , genetically modified mouse , computational biology , gene , genetics , chromosome , genome , reproductive biology , embryogenesis
Summary Thirty years after the first transgenic mouse was produced, a plethora of genetic tools has been developed to study immune cells in vivo . A powerful development is the bacterial artificial chromosome (BAC) transgenic approach, combining advantages of both conventional transgenic and knock‐in gene‐targeting strategies. In immunology the potential of BAC transgenic technology has yet to be fully harvested and, combined with a variety of elegant genetic tools, it will allow the analysis of complex immunological processes in vivo . In this short review, we discuss the applications of BACs in immunology, such as identification of regulatory regions, expression and cell‐fate mapping, cell ablation, conditional mutations and the generation of humanized mice.