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Lewis X oligosaccharides targeting to DC‐SIGN enhanced antigen‐specific immune response
Author(s) -
Wang Jingxue,
Zhang Yongmin,
Wei Jing,
Zhang Xiaoping,
Zhang Bei,
Zhu Zhenyuan,
Zou Wei,
Wang Yiqin,
Mou Zhirong,
Ni Bin,
Wu Yuzhang
Publication year - 2007
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2007.02554.x
Subject(s) - dc sign , immune system , ovalbumin , dendritic cell , elispot , antigen , biology , immunology , secretion , interferon , t cell , microbiology and biotechnology , chemistry , biochemistry
Summary Dendritic cell‐specific intercellular‐adhesion‐molecule‐grabbing non‐integrin (DC‐SIGN) is a potential target receptor for vaccination purposes. In the present study, we employed Lewis X (Le x ) oligosaccharides, which mimic natural ligands, to target ovalbumin (OVA) to human dendritic cells (DCs) via DC‐SIGN, to investigate the effect of this DC‐SIGN‐targeting strategy on the OVA‐specific immune response. We demonstrated that Le x oligosaccharides could enhance the OVA‐specific immune response as determined by enzyme‐linked immunospot assay (ELISPOT), intracellular interferon‐γ staining and 51 Cr‐release assay. An almost 300‐fold lower dose of Le x ‐OVA induced balanced interferon‐γ‐secreting cells compared to OVA alone. Furthermore, secretion of interleukin‐10, a reported mediator of immune suppression related to DC‐SIGN, was not increased by Le x ‐OVA, either alone or together with sCD40L‐stimulated groups. A blocking antibody against DC‐SIGN (12507) reduced the numbers of interferon‐γ‐secreting cells during Le x ‐OVA stimulation, yet it did not prevent Le x oligosaccharides from promoting the secretion of interleukin‐10 that was induced by ultra‐pure lipopolysaccharide. These results suggested that the strategy of DC‐SIGN targeting mediated by Le x oligosaccharides could promote a T‐cell response. This DC‐targeting may imply a novel vaccination strategy.