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Impact of T‐cell receptor Vβ haplotypes on the development of dermatitis in DS‐ Nh mice: synergistic production of interleukin‐13 caused by staphylococcal enterotoxin C and peptide glycans from Staphylococcus aureus
Author(s) -
Yoshioka Takeshi,
Imura Kinichi,
Hikita Ichiro,
Hirasawa Tsutomu,
Sakata Tsuneaki,
Matsutani Takaji,
Horikawa Tatsuya,
Arimura Akinori
Publication year - 2007
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2007.02536.x
Subject(s) - t cell receptor , biology , immunology , tlr2 , t cell , enterotoxin , chemistry , gene , immune system , innate immune system , biochemistry , escherichia coli
Summary Although the pathogenic role of interleukin‐13 (IL‐13) is a key for atopic dermatitis (AD), the mechanism of IL‐13 production in AD remains unclear. To investigate the role of the T‐cell receptor Vβ (TCR Vβ) haplotype in the development of dermatitis and the production of IL‐13 in the naturally occurring dermatitis model by staphylococcal enterotoxin C (SEC)‐producing Staphylococcus aureus , we raised DS‐ Nh mice harbouring the TCR Vβ a haplotype with a central deletion in the TCRBV gene segments, including TCR Vβ8S2. Observation and histopathological analysis of the two mouse substrains with spontaneous dermatitis indicated that later onset and weaker severity of AD‐like dermatitis were identified in mice with TCR Vβ a compared to those with TCR Vβ b . Immunohistochemical examination revealed the infiltration of a large number of CD4‐bearing T cells in the skin lesions in mice with TCR Vβ b but not in those with TCR Vβ a . Interestingly, much lower levels of serum IL‐13 were detected in mice with the TCR Vβ a than in those with the TCR Vβ b haplotype. In vitro , synthetic ligands (Pam 2 CSK4) of toll‐like receptor 2 (TLR2) synergistically produced IL‐13 with SEC in splenocytes of mice with TCR Vβ b but not of those with TCR Vβ a , and natural killer T cells were essential for this synergism. Our findings suggested that this TCR Vβ‐haplotype‐dependent synergism with TLR2 plays an important role in the development of AD‐like dermatitis in DS‐ Nh mice.