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Cytotoxic herpes simplex type 2‐specific, DQ0602‐restricted CD4 + T‐cell clones show alloreactivity to DQ0601
Author(s) -
Reichstetter Sandra,
Standifer Nathan E.,
Geubtner Kelly A.,
Liu Andrew W.,
Agar Stacy L.,
Kwok William W.
Publication year - 2006
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2005.02308.x
Subject(s) - cytotoxic t cell , biology , cd8 , t cell receptor , major histocompatibility complex , t cell , virology , streptamer , transplantation , microbiology and biotechnology , immunology , antigen , genetics , immune system , in vitro , medicine , surgery
Summary Alloreactivity is one of the most serious problems in organ transplantation. It has been hypothesized that pre‐existing alloreactive T cells are actually cross‐reacting cells that have been primed by the autologous major histocompatibility complex (MHC) and a specific peptide. CD8 + cytotoxic T lymphocytes that are alloreactive and recognize a virus‐peptide that is presented by the autologous MHC have been reported. Here we demonstrate a cross‐reactivity that exists between DQ0602 restricted, herpes simplex type 2 VP16 40–50 specific CD4 + T‐cell clones, which can be alloreactive to DQ0601. Though most of the DQ0602 restricted T‐cell clones we isolated from two different donors were not alloreactive, weakly cross‐reacting T‐cell clones could be isolated from both donors. Two strongly cross‐reacting T‐cell clones with high affinity interaction of their T‐cell receptor (TCR) with both DQ0602/VP16 40–50 and DQ0601 could be isolated from one donor. DNA sequencing of the a fragment of the Vβ gene used in their TCR confirmed that these two T cells indeed are two independent clones. These clones are cytotoxic and produce cytokines of a T helper 2‐like pattern. Possible implications in a DR‐matched transplantation setting are discussed.