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Costimulation of T‐cell proliferation by anti‐ l ‐selectin antibody is associated with the reduction of a cdk inhibitor p27
Author(s) -
Nishijima Kenichi,
Ando Munetoshi,
Sano Shusuke,
HayashiOzawa Aiko,
Kinoshita Yoshinori,
Iijima Shinji
Publication year - 2005
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2005.02234.x
Subject(s) - antibody , cd28 , mapk/erk pathway , cyclin dependent kinase , microbiology and biotechnology , t cell , cell growth , kinase , chemistry , biology , cell , cell cycle , immune system , immunology , biochemistry
Summary In this study, we investigated the costimulatory activity of l ‐selectin in primary mouse T cells. Proliferation induced by immobilized anti‐CD3 antibody was enhanced by immobilized anti‐ l ‐selectin antibody. In contrast to the anti‐CD28 antibody, anti‐ l ‐selectin antibody did not enhance interleukin‐2 (IL‐2) expression. One of the cyclin‐dependent kinase (cdk) inhibitors, p27, was reduced by costimulation with anti‐ l ‐selectin antibody, as with anti‐CD28 antibody, suggesting that the enhancement of T‐cell proliferation is the result of a reduced p27 level. Since anti‐ l ‐selectin antibody enhanced the activation of extracellular signal‐regulated protein kinase (ERK) induced by anti‐CD3 antibody, ERK plays an important role in signal integration during costimulation. These results suggest that the mechanism of T‐cell costimulation is at least partially different between CD28 and l ‐selectin, although the two mechanisms share a common downstream event, a reduction of p27 level, as a critical biochemical event in the cell cycle progression of T cells.