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B‐cell reconstitution by transplantation of B220 + CD117 + B‐lymphoid progenitors into irradiated mice
Author(s) -
Kawaguchi Susumu
Publication year - 2005
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2005.02108.x
Subject(s) - transplantation , progenitor cell , bone marrow , biology , haematopoiesis , immunology , lymphopoiesis , stem cell , microbiology and biotechnology , cd19 , b cell , andrology , medicine , antibody
Summary Bone marrow cells of 4‐week‐old CBA/J mice were searched for progenitors that can reconstitute CBA‐type B lineage (B220 + ) cells after transplantation into irradiated (BALB/c × CBA/J) F 1 mice. In recipients of B lineage‐committed B220 + CD117 + cells, which were CD19 + CD127 + CD25 – IgM – , numbers of CBA‐type B220 + cells had increased greatly by day 8 after transplantation. This increase stopped by day 10 but cell numbers remained at this level for at least 8 weeks. B‐cell production in the bone marrow of B220 + CD117 + cell recipients occurred even 8 weeks after transplantation. Probably, the transplanted B‐lymphoid progenitors are capable of self‐renewing up to 8 weeks after transplantation. B lineage‐ uncommitted CD117 high CD71 – cells, which were B220 – and included haematopoietic stem cells, could also reconstitute B lineage bone marrow cells after transplantation. Sequential development of CD117 + , CD25 + and IgM + cells in CBA‐type B220 + bone marrow cells occurred 2–4 days faster in recipients of B220 + CD117 + progenitors than in recipients of CD117 high CD71 – progenitors, suggesting that the development of the former progenitors from the latter may take 2–4 days.