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A novel adjuvant–allergen complex, CBP–rFel d 1, induces up‐regulation of CD86 expression and enhances cytokine release by human dendritic cells in vitro
Author(s) -
Andersson Theresa N.,
Ekman Gunilla J.,
Grönlund Hans,
Buentke Eva,
Eriksson Tove L. J.,
Scheynius Annika,
Van HageHamsten Marianne,
Gafvelin Guro
Publication year - 2004
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2004.01943.x
Subject(s) - allergen , cd86 , immunology , cytokine , chemistry , adjuvant , flow cytometry , in vitro , tumor necrosis factor alpha , dendritic cell , microbiology and biotechnology , immune system , biology , t cell , allergy , biochemistry
Summary Allergen‐specific immunotherapy is commonly performed with allergen extracts adsorbed to aluminium hydroxide (alum). The undesirable effects associated with the use of alum, including granuloma formation at the site of injection and stimulation of T helper 2 (Th2) cytokine production, has generated interest in alternative allergen carriers, one being carbohydrate‐based particles (CBPs). Here, we have investigated the in vitro effects of the recombinant major cat allergen Fel d 1 (rFel d 1) coupled to CBPs (CBP–rFel d 1) on human monocyte‐derived dendritic cells (MDDCs) obtained from healthy blood donors. A majority of the CD1a + MDDCs internalized fluorescein isothiocyanate‐labelled CBP–rFel d 1, as demonstrated by flow cytometry and confocal laser‐scanning microscopy. Furthermore, an up‐regulation of the expression of the costimulatory molecule, CD86, on the MDDCs was induced by CBP–rFel d 1, but not by rFel d 1 or CBPs alone. Finally, three‐ and fourfold increases in the release of interleukin‐8 and tumour necrosis factor‐α, respectively, were observed when MDDCs were cultured in the presence of CBP‐rFel d 1. Altogether, our results indicate that the use of CBPs as an allergen carrier and adjuvant is a promising candidate for the improvement of allergen‐specific immunotherapy.