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Differential production of interleukin‐10 and interleukin‐12 in mononuclear cells from leprosy patients with a Toll‐like receptor 2 mutation
Author(s) -
Kang Tae Jin,
Yeum Chung Eun,
Kim Byoung Chul,
You EunYoung,
Chae GueTae
Publication year - 2004
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2004.01926.x
Subject(s) - tlr2 , mycobacterium leprae , peripheral blood mononuclear cell , immunology , immune system , cytokine , lepromatous leprosy , toll like receptor , biology , tumor necrosis factor alpha , interleukin , leprosy , tlr4 , innate immune system , in vitro , biochemistry
Summary Toll‐like receptor 2 (TLR2) is a key mediator of the immune response to mycobacterial infections, and mutations in TLR2 have been shown to confer susceptibility to infection with mycobacteria. This study investigated the profiles of cytokines, such as interferon (IFN)‐γ, interleukin (IL)‐10, IL‐12 and tumour necrosis factor (TNF)‐α in response to Mycobacterium leprae in peripheral blood mononuclear cells (PBMC) with the TLR2 mutation Arg677Trp, a recently reported polymorphism that is associated with lepromatous leprosy. In leprosy patients with the TLR2 mutation, production of IL‐2, IL‐12, IFN‐γ, and TNF‐α by M. leprae ‐stimulated PBMC were significantly decreased compared with that in groups with wild‐type TLR2. However, the cells from patients with the TLR2 mutation showed significantly increased production of IL‐10. There was no significant difference in IL‐4 production between the mutant and wild‐type during stimulation. Thus, these results suggest that the TLR2 signal pathway plays a critical role in the alteration of cytokine profiles in PBMC from leprosy patients and the TLR2 mutation Arg677Trp provides a mechanism for the poor cellular immune response associated with lepromatous leprosy.