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Modulation of immune responses to bovine herpesvirus‐1 in cattle by immunization with a DNA vaccine encoding glycoprotein D as a fusion protein with bovine CD154
Author(s) -
Manoj Sharmila,
Griebel Philip J.,
Babiuk Lorne A.,
Van Drunen LittelVan Den Hurk Sylvia
Publication year - 2004
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2004.01877.x
Subject(s) - cd154 , biology , immune system , fusion protein , dna vaccination , antigen , antibody , plasmid , microbiology and biotechnology , immunology , cd40 , immunization , in vitro , recombinant dna , dna , gene , genetics , cytotoxic t cell
Summary The objective of this study was to determine whether a DNA vaccine encoding bovine CD154 linked to a truncated version of bovine herpesvirus‐1 (BHV‐1) glycoprotein D (tgD‐CD154) induces enhanced tgD‐specific immune responses in cattle. In vitro characterization demonstrated that tgD and tgD‐CD154 both bind to cultured bovine B cells, whereas only tgD‐CD154 induces interleukin‐4‐dependent proliferation, suggesting that tgD‐CD154 specifically binds the CD40 receptor and induces receptor signalling. Calves were immunized with plasmid encoding either tgD or tgD‐CD154 by intradermal injection with a needle‐free device. After two immunizations, tgD‐specific immune responses were observed in both vaccinated groups and after challenge with BHV‐1 these responses further increased. Animals immunized with plasmid encoding tgD‐CD154 had significantly higher tgD‐specific serum titres of immunoglobulins G and A but significantly lower numbers of tgD‐specific interferon‐γ‐secreting cells than animals immunized with plasmid encoding tgD after BHV‐1 challenge. This suggests that the expression of an antigen as a chimeric protein with CD154 can qualitatively alter immune responses in cattle. Since we previously showed that plasmid encoding tgD‐CD154 induces significantly enhanced secondary tgD‐specific antibody responses in sheep, there appear to be interspecies differences in the immune responses induced by tgD‐CD154, which suggests that both proteins in the chimeric molecule may influence protein targeting and the induction of an immune response.