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Tumour necrosis factor‐α up‐regulates decay‐accelerating factor gene expression in human intestinal epithelial cells
Author(s) -
ANDOH A.,
FUJIYAMA Y.,
SUMIYOSHI K.,
SAKUMOTO H.,
OKABE H.,
BAMBA T.
Publication year - 1997
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.1997.00358.x
Subject(s) - tumor necrosis factor alpha , necrosis , decay accelerating factor , cancer research , gene expression , gene , biology , microbiology and biotechnology , pathology , medicine , immunology , genetics , antibody , complement system
SUMMARY The increased expression of decay‐accelerating factor (DAF) has been detected in intestinal epithelial cells at the inflamed mucosa. In this study, we examined the effects of tumour necrosis factor (TNF)‐α on DAF expression in three intestinal epithelial cell lines. DAF mRNA expression was evaluated by Northern blot analysis, and DAF protein expression was analysed by biotin labelling and immunoprecipitation. TNF‐α induced a marked increase in DAF mRNA and protein expression in HT‐29, T84 and Caco‐2 cells. In HT‐29 cells, the effects of TNF‐a on DAF mRNA accumulation were observed in a dose‐dependent manner; DAF mRNA accumulation reached a maximum at 3–6 hr, and then gradually decreased. These effects of TNF‐α required de novo protein synthesis. Messenger RNA stability studies suggested that TNF‐α partially regulated DAF gene expression by a posttranscriptional mechanism. Moreover, the combination of TNF‐α and interleukin (IL)‐4 induced an additive increase in DAF mRNA accumulation in HT‐29 and T84 cells. In human intestinal epithelial cells, TNF‐α acts as a potent inducer of DAF mRNA expression, indicating an important role for TNF‐α in the regulation of DAF expression at the inflamed mucosa.