Premium
CD27/CD70 interaction directly induces natural killer cell killing activity
Author(s) -
YANG F.C.,
AGEMATSU K.,
NAKAZAWA T.,
MORI T.,
ITO S.,
KOBATA T.,
MORIMOTO C.,
KOMIYAMA A.
Publication year - 1996
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.1996.tb00017.x
Subject(s) - interleukin 21 , interleukin 12 , effector , cytotoxic t cell , natural killer cell , lymphokine activated killer cell , janus kinase 3 , microbiology and biotechnology , flow cytometry , interleukin 15 , biology , chemistry , in vitro , biochemistry
Summary The CD27 molecule is expressed on a portion of natural killer (NK) cells as well as T and B cells. To provide the functional capacity of CD27 molecule on NK cells, we here highly purified CD3 − CD56 + NK cells by flow cytometry (purity >98%), and investigated their NK cell activity via CD27/CD70 interaction using a CD70‐transfectant by a 4h 51 Cr‐release assay. The enhancement of NK activity by purified NK cells in the presence of interleukin‐2 (IL‐2) or interleukin‐12 (IL‐12) against CD70/Nalm‐6 was not recognized as compared to against mock/Nalm‐6. However, after a coculture with the fixed CD70/300‐19, the purified NK cells increased the NK cell activity against K562, the value being 10 to 20% higher than coculture with the mock/300‐19 in the presence of IL‐2 or IL‐12. The enhancement of NK activity was blocked by the addition of anti‐CD70 monoclonal antibody (mAb). In addition, conjugation of NK cells to the target was increased by coculture with the CD70/300‐19 without increased expression of adhesion molecules. In the parallel experiment, there was no increase in the killing capacity of NK cells. These results strongly show that CD27/CD70 interaction directly enhances NK activity in the presence of IL‐2 or IL‐12 by increasing the effector and target conjugate formation, indicating that CD27/CD70 interaction plays an important role in the cytotoxic function of NK cells.