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DNA repair and replication proteins as prognostic markers in melanoma
Author(s) -
Song Liang,
Robson Tammy,
Doig Tamasin,
Brenn Thomas,
Mathers Marie,
Brown Ewan R,
Doherty Val,
Bartlett John M S,
Anderson Niall,
Melton David W
Publication year - 2013
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.2012.04362.x
Subject(s) - dna replication , dna repair , biology , topoisomerase , dna , cancer research , melanoma , dna damage , replication protein a , tissue microarray , microbiology and biotechnology , gene , genetics , dna binding protein , cancer , transcription factor
Aims: Elevated expression of DNA repair and replication genes has been reported in thick, non‐fixed primary melanomas that subsequently went on to metastasize, when compared to non‐recurrent primary tumours. This increased expression could contribute to the extreme resistance shown by melanoma to DNA‐damaging chemotherapeutics. We have investigated the hypothesis that levels of key DNA repair and replication proteins are prognostic biomarkers in melanoma. Methods and results: We used a tissue microarray containing samples from all stages of melanomagenesis to investigate the hypothesis that levels of key DNA repair and replication proteins are prognostic biomarkers in a larger, more representative and readily available set of fixed primary melanomas. High expression of topoisomerase IIα (TOP2A), that relieves torsional stress during DNA replication, and XRCC5 (Ku80), required for DNA double‐strand break repair, were associated with significantly worse survival. Conclusions: Two (XRCC5 and TOP2A) of seven DNA repair and replication proteins studied were prognostic for melanoma.