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Impact of the reactive microenvironment on the bone marrow involvement of follicular lymphoma
Author(s) -
Rajnai Hajnalka,
Bödör Csaba,
Balogh Zsófia,
Gagyi Éva,
Csomor Judit,
Krenács Tibor,
Tóth Erika,
Matolcsy András
Publication year - 2012
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.2012.04187.x
Subject(s) - bone marrow , tumor microenvironment , cd68 , cd8 , pathology , cytotoxic t cell , foxp3 , lymphoma , t cell , lymph , follicular lymphoma , immune system , cancer research , medicine , immunohistochemistry , biology , immunology , biochemistry , in vitro
Rajnai H, Bödör C, Balogh Z, Gagyi É, Csomor J, Krenács T, Tóth E & Matolcsy A (2012) Histopathology 60, E66–E75 Impact of the reactive microenvironment on the bone marrow involvement of follicular lymphoma Aims: Follicular lymphoma (FL) is associated with bone marrow (BM) involvement in approximately 40–70% of cases. Previous studies have suggested that the immune‐microenvironment of FL plays an important role in the clinical behaviour of the disease. To investigate the role of the microenvironment in BM involvement of FL, we performed immunophenotypical analysis of the reactive cell populations in lymph nodes (LN) and corresponding BM of 35 patients with FL. Microenvironment patterns of BM infiltrates were compared to the corresponding features of the LN in cases with BM manifestation, and the LN microenvironment was compared in FL cases with and without BM involvement. Methods and results: Automated image‐segmentation‐based quantitation was performed in whole digital slides of tissue microarrays of formalin‐fixed paraffin‐embedded tissue biopsies. We found significantly more CD8 + T lymphocytes, forkhead box protein 3 (FoxP3) + T lymphocytes and CD68 + macrophages and fewer PD1 + T lymphocytes in the BM than in the matching LN samples. Furthermore, we observed significantly fewer CD8 + T cells and CD68 + macrophages in cases involving the BM compared to those localized only to the LNs. Conclusions: Our study showed that different tumour cell growth in the LN and BM may generate different microenvironments, and suggested that the reduced number of cytotoxic T lymphocytes and macrophages in LNs favours BM infiltration of neoplastic cells in FL.