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Increased levels of deleted in malignant brain tumours 1 (DMBT1) in active bacteria‐related appendicitis
Author(s) -
Kaemmerer Elke,
Schneider Ursula,
Klaus Christina,
Plum Patrick,
Reinartz Andrea,
Adolf Maximilian,
Renner Marcus,
Wolfs Tim G A M,
Kramer Boris W,
Wagner Norbert,
Mollenhauer Jan,
Gassler Nikolaus
Publication year - 2012
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.2011.04159.x
Subject(s) - inflammation , biology , enterocyte , bacteria , glycoprotein , pathology , appendicitis , immunology , small intestine , microbiology and biotechnology , medicine , endocrinology , genetics , general surgery
Kaemmerer E, Schneider U, Klaus C, Plum P, Reinartz A, Adolf M, Renner M, Wolfs T G A M, Kramer B W, Wagner N, Mollenhauer J & Gassler N
(2012) Histopathology 60, 561–569
Increased levels of deleted in malignant brain tumours 1 (DMBT1) in active bacteria‐related appendicitis Aims: Deleted in malignant brain tumours 1 (DMBT1; gp340) is a secreted glycoprotein which is found in the surface lining epithelia of human small and large intestine. DMBT1 is suggested to play a role in enterocyte differentiation and surface protection from intestinal bacteria. The aim of this study was to elucidate DMBT1 expression in bacteria‐related active intestinal inflammation such as appendicitis. Methods and results: mRNA and protein levels of DMBT1 were analysed in surgical resections of 50 appendices (active inflammation: n = 25). In non‐actively inflamed appendices, inter‐individual differences in basal DMBT1 levels of enterocytes and some non‐epithelial cells were found. In active appendicitis, enterocytic DMBT1 mRNA expression was increased approximately fivefold, which was paralleled by a corresponding increase of cytoplasmic and secreted DMBT1 protein levels. Increased DMBT1 expression was predominant in enterocytes adjacent to erosive lesions or ulcers. Conclusions: Our data demonstrate that bacteria‐related active inflammation results in a sharp increase of DMBT1 levels in enterocytes. These findings substantiate the view that DMBT1 is of functional relevance for host defence and modulation of the course of intestinal bacteria‐related inflammatory responses.