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Expression and significance of stem cell markers in pulmonary sclerosing haemangioma
Author(s) -
Lin XuYong,
Fan ChuiFeng,
Dong XinJun,
Yu JuanHan,
Dai ShunDong,
Wang EnHua
Publication year - 2012
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.2011.04088.x
Subject(s) - laser capture microdissection , pathology , biology , stem cell , phenotype , microdissection , cell type , cellular differentiation , abcg2 , cuboidal cell , immunohistochemistry , stem cell marker , microbiology and biotechnology , cell , gene expression , immunology , gene , medicine , genetics , atp binding cassette transporter , transporter
Lin X‐Y, Fan C‐F, Dong X‐J, Yu J‐H, Dai S‐D & Wang E‐H
(2012) Histopathology 61, 178–185
Expression and significance of stem cell markers in pulmonary sclerosing haemangioma Aims: The two major types of cells of pulmonary sclerosing haemangioma (PSH) with the same origin show significant differences in morphological phenotype. Whether these differences are caused by their different differentiation status is still uncertain. The aim of this study was to analyse their differentiation status by detecting the expression of several stem cell markers in PSH. Methods and results: The expression of stem cell markers was examined by using streptavidin peroxidase (SP) immunohistochemisty in 45 PSH specimens. Also, the two types of cells were, respectively, captured by laser capture microdissection (LCM) from 28 PSH specimens, and total RNA was then extracted followed by reverse transcription–polymerase chain reaction (RT–PCR). The results demonstrated that the expression rates of ABCG2, Notch1 and Notch3 in polygonal cells were significantly higher than those in cuboidal cells ( P < 0.05), and the expression levels of ABCG2, Notch3 and Jagged1 in polygonal cells were clearly higher than those in cuboidal cells ( P < 0.05). Conclusion: The data obtained provided evidence that the two types of cells in PSH may be different in differentiation status. The differentiation difference between the two types of cells might lead to variation in their morphological phenotype.