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Expression of activation‐induced cytidine deaminase in ulcerative colitis‐associated carcinogenesis
Author(s) -
Gushima Masaki,
Hirahashi Minako,
Matsumoto Takayuki,
Fujita Kouhei,
Ohuchida Kenoki,
Oda Yoshinao,
Yao Takashi,
Iida Mitsuo,
Tsuneyoshi Masazumi
Publication year - 2011
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.2011.03965.x
Subject(s) - cytidine deaminase , ulcerative colitis , carcinogenesis , cancer research , activation induced (cytidine) deaminase , cytidine , colitis , medicine , biology , enzyme , immunology , somatic hypermutation , cancer , biochemistry , disease , antibody , b cell
Gushima M, Hirahashi M, Matsumoto T, Fujita K, Ohuchida K, Oda Y, Yao T, Iida M & Tsuneyoshi M (2011) Histopathology 59 , 460–469 Expression of activation‐induced cytidine deaminase in ulcerative colitis‐associated carcinogenesis Aims: Activation‐induced cytidine deaminase (AID) is a DNA/RNA‐editing enzyme that is essential for hypermutation and class‐switch recombination in immunoglobulin genes. The aim of this study was to investigate the expression of AID and its association with p53 mutation in ulcerative colitis (UC)‐associated carcinogenesis. Methods and results: The expression of AID was examined in 25 patients with UC‐associated neoplasia, 20 UC patients without neoplasia, 18 patients with non‐inflamed colorectal mucosa unaffected by UC, and 19 patients with sporadic colorectal cancer, by immunohistochemistry and quantitative reverse transcription polymerase chain reaction analysis. Mutational analysis and immunohistochemistry for p53 were also performed. The degree of AID expression was not different between UC‐associated neoplasia and sporadic colorectal cancer. However, AID was expressed in both UC‐associated neoplasia and UC without neoplasia. Whereas AID expression in UC‐associated neoplasia was not correlated with the grade of dysplasia, expression in non‐neoplastic mucosa of UC was correlated with the histological grade of inflammation. In UC‐associated neoplasia, there was no significant correlation between AID expression and p53 mutation. Conclusions: AID is associated with inflammation in UC, whereas it may not specifically contribute to carcinogenesis in UC.