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Significance of p53‐binding protein 1 nuclear foci in uterine cervical lesions: endogenous DNA double strand breaks and genomic instability during carcinogenesis
Author(s) -
Matsuda Katsuya,
Miura Shiro,
Kurashige Tomomi,
Suzuki Keiji,
Kondo Hisayoshi,
Ihara Makoto,
Nakajima Hisayoshi,
Masuzaki Hideaki,
Nakashima Masahiro
Publication year - 2011
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.2011.03963.x
Subject(s) - carcinogenesis , biology , genome instability , in situ hybridization , cervical intraepithelial neoplasia , cancer research , immunofluorescence , cervical cancer , microbiology and biotechnology , pathology , cancer , dna damage , dna , antibody , gene expression , medicine , gene , immunology , genetics
Matsuda K, Miura S, Kurashige T, Suzuki K, Kondo H, Ihara M, Nakajima H, Masuzaki H & Nakashima M
(2011) Histopathology 59 , 441–451 Significance of p53‐binding protein 1 nuclear foci in uterine cervical lesions: endogenous DNA double strand breaks and genomic instability during carcinogenesis Aims: A defective DNA damage response can result in genomic instability (GIN) and lead to transformation to cancer. As p53‐binding protein 1 (53BP1) localizes at the sites of DNA double strand breaks (DSBs) and rapidly forms nuclear foci (NF), the presence of 53BP1 NF can be considered to be an indicator of endogenous DSBs reflecting GIN. Our aim was to analyse the presence of DSBs by immunofluorescence for 53BP1 expression in a series of cervical lesions, to evaluate the significance of GIN during carcinogenesis. Methods and results: A total of 80 archival cervical tissue samples, including 11 normal, 16 cervical intraepithelial neoplasia (CIN)1, 15 CIN2, 24 CIN3 and 14 squamous cell carcinoma samples, were analysed for 53BP1 NF, human papillomavirus (HPV) infection, and p16 INK4a overexpression. The number of 53BP1 NF in cervical cells appeared to increase with progression during carcinogenesis. The distribution of 53BP1 NF was similar to that of the punctate HPV signals as determined by in‐situ hybridization and also to p16 INK4a overexpression in CIN, suggesting an association with viral infection and replication stress. Conclusions: Immunofluorescence analysis of 53BP1 expression can be a useful tool with which to estimate the level of GIN. During cervical carcinogenesis, GIN may allow further accumulation of genomic alterations, causing progression to invasive cancer.