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RNA‐binding protein LIN28 is a sensitive marker of ovarian primitive germ cell tumours
Author(s) -
Xue Debin,
Peng Yan,
Wang Fenghua,
Allan Robert W,
Cao Dengfeng
Publication year - 2011
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.2011.03949.x
Subject(s) - lin28 , germ cell tumors , germ cell , pathology , immunohistochemistry , staining , biology , teratoma , immature teratoma , embryonic stem cell , medicine , genetics , chemotherapy , induced pluripotent stem cell , gene
Xue D, Peng Y, Wang F, Allan R W & Cao D
(2011) Histopathology 59 , 452–459 RNA‐binding protein LIN28 is a sensitive marker of ovarian primitive germ cell tumours Aims: LIN28 is an RNA‐binding protein that has been detected in testicular germ cell tumours (GCTs), but its status in ovarian GCTs is unknown. The aim was to determine the immunohistochemical profile of LIN28 in ovarian GCTs. Methods and results: Immunohistochemistry of LIN28 was performed in 110 primary and 11 metastatic ovarian GCTs. The percentage of tumour cells stained was scored as 0, 1+ (1–30% cells), 2+ (31–60%), 3+ (61–90%), and 4+ (>90%). To determine its specificity, we stained LIN28 in 119 non‐GCTs, including 37 clear cell carcinomas. Strong 4+ LIN28 staining was seen in 4/4 (100%) gonadoblastomas, 7/7 (100%) embryonal carcinomas (ECs), and 41/41 (100%) yolk sac tumours (YSTs). Among 39 dysgerminomas, 4+ staining was seen in 37 and 3+ staining in two (strong in 37; mixed weak and strong in two). Twelve of 14 immature teratomas showed variable LIN28 staining (1+ to 4+) in the immature neuroepithelium (weak to strong staining), whereas mature teratomas, carcinoids, struma ovarii and strumal carcinoids were negative. Only 5/117 non‐GCTs (1/37 clear cell carcinomas) showed weak to moderate 1–2+ staining. Conclusions: LIN28 is a sensitive marker for gonadoblastomas, dysgerminomas, ECs, and YSTs. LIN28 can be used to distinguish them from non‐GCTs.