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Immunohistochemical detection of arginine methylated proteins (MeRP) in archival tissues
Author(s) -
Vezzalini Marzia,
Aletta John M,
Beghelli Stefania,
Moratti Elisabetta,
Della Peruta Marco,
Mafficini Andrea,
Mojica Wilfrido D,
Mombello Aldo,
Scarpa Aldo,
Sorio Claudio
Publication year - 2010
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.2010.03684.x
Subject(s) - immunohistochemistry , polyclonal antibodies , antibody , arginine , chemistry , biology , pathology , microbiology and biotechnology , biochemistry , medicine , immunology , amino acid
Vezzalini M, Aletta J M, Beghelli S, Moratti E, Della Peruta M, Mafficini A, Mojica W D, Mombello A, Scarpa A & Sorio C
(2010) Histopathology 57 , 725–733
Immunohistochemical detection of arginine methylated proteins ( MeRP ) in archival tissues Aims: To (i) determine whether methylarginine‐specific antibodies can be employed for standard immunohistochemical analysis of paraffin‐embedded tissues, (ii) analyse methylarginine expression in normal and neoplastic tissues and (iii) correlate methylarginine expression with that of protein arginine methyltransferase (PRMT1), the predominant cellular arginine methyltransferase. Methods and results: Immunohistochemistry of normal and cancer tissues was performed utilizing three commercial polyclonal antibodies: anti‐methylarginine‐specific antibody (anti‐mRG) raised against a methylarginine peptide, Control antibody (anti‐RG), a control antiserum raised against a corresponding arginine peptide without any methylated residues and anti‐PRMT1. Nuclear and/or cytoplasmic methylarginine expression was detected in all keratinized and non‐keratinized epithelia. A preliminary survey of a series of thyroid, pancreatic, colonic and gastric cancers identified a different pattern of methylarginine expression in comparison with normal tissue. A correlation between methylarginine staining and PRMT1 expression was found in all normal and cancer tissues analysed. Conclusion: Methylarginine‐specific antibodies are capable of recognizing methylarginine proteins (MeRP) in paraffin‐embedded tissues. Methylarginine proteins are expressed widely and show differences in subcellular localization in various organs and neoplastic conditions. The efficient detection of methylproteins by standard immunohistochemistry provides a new tool to investigate the role of methylarginine proteins (MeRP) in biological processes including carcinogenesis.