Premium
Immunohistochemical expression of p16 INK4A protein as a helpful marker of a subset of potentially malignant oral epithelial lesions: study on a series with long‐term follow‐up
Author(s) -
Montebugnoli Lucio,
Cervellati Fabio,
Cocchi Roberto,
Farnedi Anna,
Pennesi Maria Gabriella,
Flamminio Federica,
Foschini Maria Pia
Publication year - 2010
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.2010.03671.x
Subject(s) - immunohistochemistry , pathology , dysplasia , epithelial dysplasia , biopsy , malignant transformation , staining , cancer , basal cell , medicine , anatomical pathology , histopathology , carcinoma , ki 67 , biology
Montebugnoli L, Cervellati F, Cocchi R, Farnedi A, Pennesi M G, Flamminio F & Foschini M P
(2010) Histopathology 57 , 528–534
Immunohistochemical expression of p16 INK4A protein as a helpful marker of a subset of potentially malignant oral epithelial lesions: study on a series with long‐term follow‐up Aim: To examine a group of lesions that progressed to oral squamous cell carcinoma (OSCC) to determine whether p16 INK4A expression is an early finding during malignant transformation, and whether immunohistochemical evaluation of p16 INK4A is an appropriate prognostic marker. Methods and results: Twenty cases of OSCC were investigated. All cases had had a biopsy on the same site as OSCC performed at least 1 year before OSCC (range 1–11 years; mean 3.15 ± 3.1 years). Twenty specimens from normal oral mucosa served as controls. p16 INK4A expression was evaluated by immunohistochemical analysis and cases showing >5% of stained cells were defined as ‘positive’. All 20 control cases were negative for p16 INK4A . Oral lesions were p16 INK4A ‐positive in nine cases and negative in 11. No significant relationship was found between p16 INK4A positivity and the presence/absence of dysplasia. Among OSCC, nine tumours showed p16 INK4A positivity and 11 showed negativity. A significant relationship (χ 2 = 7.1; P < 0.01) was found between the presence/absence of p16 INK4A staining in OSCC and the presence/absence of p16 INK4A staining in lesions preceding OSCC. Conclusions: p16 INK4A immunohistochemistry has a potential role in detecting a subset of p16 INK4A ‐positive lesions with malignant potential.