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Genomic and immunophenotypical differences between hepatocellular carcinoma with and without cirrhosis
Author(s) -
Tretiakova Maria S,
ShabaniRad Meer T,
Guggisberg Kelly,
Hart John,
Anders Robert A,
Gao Zuhua
Publication year - 2010
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.2010.03554.x
Subject(s) - hepatocellular carcinoma , cyclin d1 , cirrhosis , cancer research , pathogenesis , immunohistochemistry , biology , wnt signaling pathway , pathology , carcinoma , tissue microarray , retinoblastoma , cell cycle , medicine , cancer , gene , genetics
Tretiakova M S, Shabani‐Rad M T, Guggisberg K, Hart J, Anders R A & Gao Z‐h
(2010) Histopathology 56, 683–693
Genomic and immunophenotypical differences between hepatocellular carcinoma with and without cirrhosis Aims: To compare the expression of genes involved in p53, Wnt/β‐catenin, and retinoblastoma (Rb) 1 pathways between cirrhosis‐associated hepatocellular carcinoma (HCC‐C) and hepatocellular carcinoma arising in non‐cirrhotic liver (HCC‐NC). Methods and results: The gene expression profile was analysed using oligo‐DNA arrays, and then validated at protein level in a tissue microarray using immunohistochemistry. Compared with their background non‐neoplastic liver tissue, HCC‐C showed a significantly higher rate of p53, β‐catenin (protein only) and cyclin D1 expression, whereas HCC‐NC showed a significantly higher rate of p21 Waf1/cip1 and p27 Kip1 expression. HCC‐C had a significantly higher rate of p53 expression and a significantly lower rate of p21 waf1/cip1 expression than HCC‐NC. There was no statistically significant association between the expression of genetic markers and tumour histological grade, underlying aetiology, or lymphovascular invasion. Aberrant β‐catenin expression was more commonly seen in single tumours in comparison with multiple tumours. Increased p16 INK4 and p21 waf1/cip1 expression was more commonly observed in large‐sized tumours (>50 mm) than small‐sized tumours. Conclusions: Alteration of the p53 pathway plays a more important role in the pathogenesis of HCC‐C, whereas alterations in cell cycle regulators p21 waf1/cip1 and p27 Kip1 play a more important role in the pathogenesis of HCC‐NC.