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Hyalinizing trabecular tumour of the thyroid—differential expression of distinct miRNAs compared with papillary thyroid carcinoma
Author(s) -
Sheu SienYi,
Vogel Esther,
Worm Karl,
Grabellus Florian,
Schwertheim Suzan,
Schmid Kurt Werner
Publication year - 2010
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.2010.03526.x
Subject(s) - thyroid carcinoma , thyroid , microrna , pathology , differential diagnosis , immunohistochemistry , carcinoma , cancer research , gene , biology , endocrinology , medicine , genetics
Sheu S‐Y, Vogel E, Worm K, Grabellus F, Schwertheim S & Schmid K W
(2010) Histopathology 56 , 632–640 Hyalinizing trabecular tumour of the thyroid—differential expression of distinct miRNAs compared with papillary thyroid carcinomaAims: To compare the expression pattern of five microRNAs (miRNAs) (146b, ‐181b, ‐21, ‐221, ‐222) of papillary thyroid carcinoma (PTC) and hyalinizing trabecular tumour of the thyroid (HTT). Methods and results: The expression pattern of five miRNAs known to be up‐regulated in PTC was retrospectively analysed in 18 HTTs, adjacent normal thyroid tissue, 10 PTCs, 10 follicular adenomas and 10 non‐toxic multinodular goitres (MNG) by reverse transcriptase‐polymerase chain reaction using the TaqMan miRNA assay. Furthermore, the two common genetic alterations characteristic for PTC, the V600E mutation of the BRAF gene and RET/PTC 1 and 3 rearrangements, were determined in all HTTs. All miRNAs were significantly up‐regulated in PTCs, whereas all miRNAs in HTT, normal thyroid tissue, adenomas, and MNGs were down‐regulated. Calculating relative changes in gene expression, a 510‐fold change of miRNA 146b between PTC and HTT could be observed followed by fold changes between 6.4 and 29 in the remaining miRNAs ( P < 0.001). All HTTs lacked BRAF mutations and RET/PTC rearrangements. Conclusions: Our findings do not support the concept that a high proportion of HTT represents a variant of PTC. It is suggested that HTTs lacking both a miRNA expression pattern characteristic for PTC and RET/PTC rearrangements are re‐designated as ‘hyalinizing trabecular adenomas’.