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Differences in genetic instability and cellular phenotype among Barrett’s, cardiac, and gastric intestinal metaplasia in a Japanese population with Helicobacter pylori
Author(s) -
Watari Jiro,
Moriichi Kentaro,
Tanabe Hiroki,
Sato Ryu,
Fujiya Mikihiro,
Miwa Hiroto,
Das Kiron M,
Kohgo Yutaka
Publication year - 2009
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.2009.03370.x
Subject(s) - microsatellite instability , intestinal metaplasia , helicobacter pylori , gastroenterology , loss of heterozygosity , medicine , population , incidence (geometry) , pathogenesis , metaplasia , biology , allele , microsatellite , genetics , gene , physics , environmental health , optics
Aims: Intestinal metaplasia is considered to be a precursor lesion in both Barrett’s and intestinal‐type gastric cancer. The aim was to clarify the differences in molecular pathology between specialized intestinal metaplasia (SIM) in Barrett’s oesophagus (BO), cardiac (CIM) and gastric intestinal metaplasia (GIM). Methods and results: Eighty‐eight SIM cases with BO, 30 CIM cases and 52 GIM cases in patients with or without Helicobacter pylori infectionwere analysed for genetic instability and Das‐1. Microsatellite instability and a loss of heterozygosity were evaluated at five microsatellite loci. The incidence of genetic instability was 55.7% in SIM, 40.0% in CIM and 23.1% in GIM, revealing a significant difference between SIM and GIM ( P < 0.0005). For each microsatellite marker analysed, there were obvious differences in frequency among the three conditions. Das‐1 reactivity was significantly higher in SIM than in CIM or GIM ( P < 0.0001, both). Interestingly, both genetic instability and Das‐1 reactivity in SIM showed a significantly higher incidence in patients with H. pylori infection than in those without ( P < 0.005 and P < 0.01, respectively). Conclusions: SIM is distinct from CIM and GIM, and the pathogenesis of SIM, like that of GIM, is associated to some degree with H. pylori infection in a Japanese population.