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Calcitonin receptor immunoreactivity associated with specific cell types in diseased radial and internal mammary arteries
Author(s) -
Wookey P J,
Zulli A,
Buxton B F,
Hare D L
Publication year - 2008
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.2008.02979.x
Subject(s) - vasa vasorum , immunohistochemistry , pathology , receptor , endothelium , mammary artery , parenchyma , biology , cell type , tunica media , antibody , anatomy , calcitonin , cell , medicine , endocrinology , artery , smooth muscle , immunology , genetics
Aims: To determine and quantify calcitonin receptor (CTR) immunoreactivity associated with specific cell types within, and associated with, the endothelial layers, neo‐intima, media and vasa vasorum of diseased radial and internal mammary arteries. Methods and results: Immunohistochemistry and anti‐CTR antibodies were used to identify positive cells within remnants of diseased human radial ( n = 3) and internal mammary arteries ( n = 4) that remained after bypass surgery. Three cell types expressed CTR, including endothelial cells, fibroblast‐like cells within the neo‐intima, and cellular structures aligned with the smooth muscle cells of the media. Other smaller cells within the surrounding parenchyma of the vasa vasorum of diseased vessels and blood‐borne cells were also immunoreactive. Immunoquantification of CTR expression (Intensity × Proportional Area) in the endothelium ( P < 0.05), neo‐intima ( P < 0.02) and media ( P < 0.03) established a significant statistical correlation (Students’ two‐tailed t ‐test) with the ratio of intimal/media thickness. Conclusions: Increased immunoreactivity developed using anti‐CTR antibodies was associated with specific cell types in the endothelial layers, neo‐intima, media and vasa vasorum of diseased regions of radial and internal mammary arteries, in which there was an increased intimal/media ratio. Furthermore, CTR+, blood‐borne cells present in the vessels of diseased regions suggest recruitment into these surrounding tissues.