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Diagnostic utility of FLI‐1 monoclonal antibody and dual‐colour, break‐apart probe fluorescence in situ (FISH) analysis in Ewing's sarcoma/primitive neuroectodermal tumour (EWS/PNET). A comparative study with CD99 and FLI‐1 polyclonal antibodies
Author(s) -
MhawechFauceglia P,
Herrmann F,
Penetrante R,
Beck A,
Sait S,
Block A M,
Odunsi K,
Fisher J,
Balos L,
Cheney R T
Publication year - 2006
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.2006.02535.x
Subject(s) - cd99 , ewing's sarcoma , fluorescence in situ hybridization , immunohistochemistry , sarcoma , primitive neuroectodermal tumor , pathology , fish <actinopterygii> , polyclonal antibodies , biology , neuroectodermal tumor , monoclonal antibody , medicine , antibody , immunology , genetics , vimentin , fishery , chromosome , gene
Aims: To compare the sensitivity and specificity of the recently commercially available FLI‐1 monoclonal (FLI‐1m) antibody with the currently used antibodies [CD99 and FLI‐1 polyclonal (FLI‐1p)] in the diagnosis of Ewing's sarcoma/primitive neuroectodermal tumour (EWS/PNET) and to determine the diagnostic value of the EWSR1 (22q12) dual‐colour, break‐apart rearrangement probe fluorescence in situ hybridization (FISH) technique. Materials and methods: Forty‐three cases of well‐documented EWS/PNET and 15 non‐EWS/PNET cases were retrieved from the archival files. Immunohistochemistry (IHC) for FLI‐1p, FLI‐1m and FISH analysis was performed. Results: The most sensitive and specific test panel for the diagnosis of EWS/PNET is the combination of CD99 and FLI‐1p. FISH had a very high specificity (100%) but only a moderate sensitivity (50%). Conclusion: The combination of CD99 and FLI‐1p is the method of choice for the diagnosis of EWS/PNET. EWRS1 (22q12) dual‐colour, break‐apart rearrangement probe FISH should be used as a confirmatory test in addition to CD99 and FLI1‐p due to its high specificity.