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Tissue microarray analysis of multiple gene expression in intestinal metaplasia, dysplasia and carcinoma of the stomach
Author(s) -
Sun Y,
Li JY,
He JS,
Zhou LX,
Chen K
Publication year - 2005
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.2005.02111.x
Subject(s) - dysplasia , intestinal metaplasia , mucin , metaplasia , pathology , tissue microarray , carcinoma , cyclin e , carcinogenesis , immunohistochemistry , cancer research , biology , stomach , malignant transformation , cyclin , medicine , cancer , cell cycle , genetics
Aims : To study multiple gene expression patterns and their roles in the process of gastric carcinogenesis. Methods and results : Using a high‐throughput tissue microarray technique, 169 specimens from gastric carcinomas, precursor lesions and normal mucosa were immunostained on a series of tissue chips for p53, p21 WAF1/CIP1 cyclin E, Bcl‐2, c‐met and mucin 5AC expression. The overexpression of p53 was observed in 10.7% of low‐grade dysplasia (LGD), 38.1% of high‐grade dysplasia (HGD) and 39.6% of intestinal type gastric carcinoma (IGC). Expression of p21 WAF1/CIP1 was found in 47.6% of incomplete intestinal metaplasia (IM), 36.7% of dysplasia (Dys) and 29.5% of IGC. The overexpression of cyclin E was more frequently present in carcinomas than in Dys ( P < 0.05); moreover, high‐level expression (>25% in extent) of cyclin E was observed only among IGC. Abnormal Bcl‐2 expression was present in 81.0% of incomplete IM, 69.4% of Dys and 22.9% of IGC. Along with progression of the lesion, the expression of c‐met increased; in contrast, mucin 5AC decreased gradually. Conclusions : The specific expression pattern in incomplete IM was mucin 5AC(+)/Bcl‐2(+)/p53(–)/cyclin E(–), while mucin 5AC(–)/cyclin E(+) was specific for IGC. p53 was useful for distinguishing LGD from HGD. High‐level expression of cyclin E might be an indicator for malignant transformation of dysplasia.