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The pattern and frequency of t(14;18) translocation and immunophenotype in Asian follicular lymphoma
Author(s) -
Peh S C,
Shaminie J,
Tai Y C,
Tan J,
Gan S S
Publication year - 2004
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.2004.01979.x
Subject(s) - chromosomal translocation , follicular lymphoma , immunophenotyping , lymphoma , follicular phase , immunohistochemistry , fluorescence in situ hybridization , pathology , biology , population , immunology , medicine , gene , genetics , flow cytometry , environmental health , chromosome
Aims: Follicular lymphoma is frequently associated with t(14;18)(q32;q21) translocation. This study was undertaken to determine the pattern of Bcl‐2, CD10 and Bcl‐6 expression in relation to t(14;18) translocation in follicular lymphoma from a cohort of a multi‐ethnic Asian population. Methods and results: Sixty‐two cases of follicular lymphoma were retrieved for immunohistochemistry, and t(14;18) translocation analysis by polymerase chain reaction and fluorescent in‐situ hybridization techniques. Bcl‐2 expression was present in 74% of the cases. CD10 expression was also relatively low (61%), with decreasing frequency of expression in high‐grade tumours. Bcl‐6 protein was expressed in most of the tumours (88%) regardless of the tumour grade. The t(14;18) translocation was detected in 46 cases (74%) with an extremely high rate of t(14;18) translocation in ethnic Indian cases (100%). Conclusion: The frequency of t(14;18) translocation in this series of follicular lymphomas was higher when compared with previous Asian reports, but in accordance with European and North American findings. CD10 expression is strongly associated with a t(14;18) translocation event, but the overall CD10 expression was relatively low, possibly due to the high proportion of high‐grade tumours in the series. t(14;18) translocation was not associated with Bcl‐2 or Bcl‐6 expression.