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Distribution of the cdc2 gene product in normal tissues: an immunocytochemical study using four new monoclonal antibodies
Author(s) -
DOUSSISANAGNOSTOPOULOU I.A.,
TENNANT R.C.,
GAN J.,
GATTER K.C.
Publication year - 1994
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.1994.tb00534.x
Subject(s) - cyclin dependent kinase 1 , biology , monoclonal antibody , antibody , cell cycle , microbiology and biotechnology , immunohistochemistry , epithelium , pathology , cell , immunology , medicine , genetics
The reproductive cycle in eukaryotic cells is partly controlled by the p34 protein kinase, the product of the cdc2 gene. We report here the tissue reactivity of four new anti‐cdc2 monoclonal antibodies in relation to the known proliferation markers Ki‐67 and JC1. In tissues where proliferation occurs, germinal centres in the tonsil, basal layers of tonsular epithelium and skin, cortex of the thymus, seminiferous tubules of the testis and epithelium of the colon, the anti‐cdc2 antibodies gave positive nuclear staining as did the proliferation markers. The percentage of positive cells was, however, lower with the cdc2 antibodies. Given the role of the cdc2 gene at specific points of the cell cycle, these antibodies are potentially useful as markers of different phases of the cell cycle and may help to detect abnormalities in cell cycle control in disease.