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Lymphocyte predominance Hodgkin's disease (nodular paragranuloma)–a bcl‐2 negative germinal centre lymphoma
Author(s) -
GARA P.A.,
MARTINEZ P.,
SANCHEZ L.,
VILLUENDAS R.,
ORRADRE J.L.,
OLIVA H.,
PIRIS M.A.
Publication year - 1991
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.1991.tb00896.x
Subject(s) - germinal center , lymphoma , biology , breakpoint , pathology , immunophenotyping , lymphocyte , monoclonal antibody , immunoglobulin heavy chain , monoclonal , b cell , immunology , chromosomal translocation , antibody , antigen , medicine , genetics , gene
Hodin's disease, lymphocyte predominance type (nodular paragranuloma), is of germinal centre origin and the tumours cells have a B‐cell phenotype. As the t(14;18) translocation, and the subsequent expression of bcl‐2 protein by germinal centre cells, is the most characteristic finding of centroblastic‐centrocytic lymphoma, we have tested a series of 11 cases of lymphocyte predominance Hodgkin's disease, using Southern blot analysis for the major breakpoint region and the minor breakpoint cluster region, polymerase chain reaction with primers for the major and minor breakpoint cluster region, and immunohistological studies with a monoclonal antibody specific for the bcl‐2 protein. All three techniques gave negative results in the cases of Hodgkin's disease, establishing a clear differentiation from centroblastic‐centrocytic lymphoma. These findings are useful in the differential diagnosis between the two entities and raise the question of the non‐clonal nature of lymphocyte predominance Hodgkin's disease.