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Vimentin expression of renal cell carcinoma in relation to DNA content and histological grading: a combined light microscopic, immunocytochemical and cytophotometrical analysis
Author(s) -
DIERICK A.M.,
PRAET M.,
ROELS H.,
VERBEECK P.,
ROBYNS C.,
OOSTERLINCK W.
Publication year - 1991
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.1991.tb00852.x
Subject(s) - vimentin , pathology , cytokeratin , mitotic index , biology , pleomorphism (cytology) , immunohistochemistry , clear cell , grading (engineering) , renal cell carcinoma , carcinoma , mitosis , medicine , microbiology and biotechnology , ecology
An immunocytochemical and cytophotometrical analysis of 30 renal cell carcinomas was carried out in order to investigate the expression of cytokeratin and vimentin in relation to the ploidy and mitotic index. Thirty renal cell carcinomas were studied using monoclonal antibodies CAM 5.2 and anti‐vimentin in a biotin‐streptavidin staining procedure. The renal cell carcinomas were classified according to the criteria of Furhman with a nuclear grading from I to IV. All carcinomas expressed cytokeratin, whereas co‐expression with vimentin was present only in 53%. Expression for both intermediate filaments was present in aneuploid tumours with a marked nuclear pleomorphism and was more frequently related to a tubulo‐papillary and pseudosarcomatous growth pattern. It is suggested that vimentin expression in renal cell carcinomas may be useful as a prognostic index in addition to nuclear grade, DNA content, mitotic rate and tumour stage.