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Immunohistochemical detection of the multidrug transport protein P1 70 in human normal tissues and malignant lymphomas
Author(s) -
PILERI S.A.,
SABATTINI E.,
FALINI B.,
TAZZARI P.L.,
GHERLINZONI F.,
MICHIELI M.G.,
DAMIANI D.,
ZUCCHINI L.,
GOBBI M.,
TSURUO T.,
BACCARANI M.
Publication year - 1991
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.1991.tb00003.x
Subject(s) - immunohistochemistry , pathology , stroma , biology , monoclonal antibody , antibody , staining , bone marrow , p glycoprotein , lymphoma , pancreas , multiple drug resistance , medicine , immunology , endocrinology , microbiology and biotechnology , antibiotics
Two monoclonal antibodies, MRK16 and C219, both directed at the 170 kDa P‐glycoprotein multidrug resistance agent, were applied to frozen sections or cytospin preparations from normal human tissues and 60 non‐Hodgkin's malignant lymphomas. Adrenal gland, kidney, liver and pancreas were always stained by the reagents, albeit with slightly different patterns. Brain capillaries as well as macrophages and some elements of the bone marrow, peripheral blood, ovarian stroma and colonic, gastric and jejunal mucosa were positive in all examined preparations. There were differences in the staining patterns with the two antibodies. Among the 60 non‐Hodgkin's lymphomas, 25 contained a number of positive cells, which ranged from 2% to 100%. No correlation was seen between the expression of P1 70 and histological type, stage, clinical symptoms or growth fraction. A close relationship was shown between the presence of P1 70 positive elements and the clinical course of the disease ( P <0.001).