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Paraffin section immunohistochemistry. II. Hodgkin's disease and large cell anaplastic (Ki1) lymphoma
Author(s) -
HALL P. A.,
D'ARDENNE A. J.,
STANSFELD A. G.
Publication year - 1988
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.1988.tb02021.x
Subject(s) - immunohistochemistry , anaplastic large cell lymphoma , pathology , lymphoma , medicine , section (typography) , dermatology , advertising , business
A panel of antibodies that recognize antigens that survive fixation and conventional processing have been applied to 43 cases of Hodgkin's disease and five cases of large cell anaplastic lymphoma. Reed‐Sternberg cells in all five cases of nodular lymphocyte predominance Hodgkin's disease were positive with leucocyte common (CD45) and B‐cell antibodies, and negative with LeuM1 (CD15) and BerH2 (CD30) antibodies. In other types of Hodgkin's disease, Reed‐Sternberg cells were positive with BerH2 in all cases, positive with LeuM1 in 63% of cases (with enzymic predigestion), positive with at least one B‐cell antibody in 29% of cases and positive for CD45 in 8% of cases. In 19% of all cases, Reed‐Sternberg cells were positive for epithelial membrane antigen and in 93% they were positive with TAL1B5 (anti‐class II MHC). No case showed immunoreactivity with anti‐T‐cell antibodies. The patterns of immunoreactivity of large cell anaplastic lymphoma were similar, except that none was positive with B‐cell antibodies and three were positive with T‐cell antibodies. All five were positive with BerH2 (CD30) and TAL1B5. Comparison of the results with those seen in other cases of non‐Hodgkin's lymphoma indicates that, with the currently available reagents, this immunohistological profile cannot be used as the sole diagnostic discriminant of these conditions; this must still be based upon careful morphological assessment.