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Correlation of histopathological characteristics with staining patterns in human melanoma assessed by (monoclonal) antibodies reactive on paraffin sections
Author(s) -
HAGEN E. C.,
VENNEGOOR C.,
SCHLINGEMANN R. O.,
VELDE E. A.,
RUITER D. J.
Publication year - 1986
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.1986.tb02522.x
Subject(s) - melanoma , pathology , medicine , immunoperoxidase , staining , immunohistochemistry , mitotic index , monoclonal antibody , antibody , mitosis , biology , immunology , cancer research , microbiology and biotechnology
In order to investigate their possible role as prognostic markers, staining with the antibodies NKI/C‐3 and anti‐S100, that are applicable on paraffin sections, was examined using a group of primary cutaneous melanomas and autologous metastases using the immunoperoxidase procedure. All melanoma lesions stained with anti‐S100, and the large majority with NKI/C‐3. In primary melanomas showing a moderate or dense associated lymphocytic infiltrate, significantly more tumour cells stained with anti‐S100 than in primary melanomas with a slight or absent infiltrate. In markedly pigmented metastases, significantly more tumour cells stained with NKI/C‐3 than in less pigmented lesions; in primary melanomas this phenomenon just failed to be significant. In metastases with a high mitotic index a significantly lower proportion of tumour cells stained with NKI/C‐3 than in lesions with a low mitotic index. No significant differences in staining were found between a group of primary melanomas with metastases and a group without metastases within a follow‐up period of 5 years. Therefore, although staining with NKI/C‐3 and anti‐S100 appears to be associated with certain histopathological characteristics, it has no direct contribution to the assessment of prognosis in primary melanoma.