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Plastic embedding in routine histology I: Preparation of semi‐thin sections of undecalcified marrow cores
Author(s) -
ISLAM A.,
FRISCH BERTHA
Publication year - 1985
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.1985.tb02809.x
Subject(s) - bone decalcification , bone marrow , histology , pathology , stain , giemsa stain , biomedical engineering , histopathology , materials science , biopsy , medicine , staining
The preparation of sections of bone marrow cores in a routine histology laboratory requires decalcification and paraffin embedding, which produces shrinkage and considerable loss of cellular detail. This may be avoided by using plastic embedding procedures. This report describes a simplified routine procedure for using methyl‐methacrylate as a plastic embedding medium for the preparation of semi‐thin sections of undecalcified bone marrow cores. A modification of the May‐Grunwald‐Giemsa stain is also given which provides good colour differentiation of various haematopoietic cells in the marrow. The method is simple, reproducible, requires no expensive equipment, and is suitable for routine processing of bone marrow biopsy cores in any histopathology laboratory.