Premium
Quantitative analysis of histological parameters in giant cell lesions of the jaws and long bones
Author(s) -
FRANKLIN C. D.,
CRAIG G. T.,
SMITH C. J.
Publication year - 1979
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1111/j.1365-2559.1979.tb03032.x
Subject(s) - haematoxylin , giant cell , stereology , anatomy , lesion , pathology , significant difference , staining , biology , medicine
Stereological techniques have been used to compare histological parameters of the giant cell component in Io giant cell tumours of long bones, 10 central giant cell granulomas of the jaws and 10 peripheral giant cell granulomas of the jaws. The giant cell parameters investigated were: mean profile diameter, mean diameter, mean profile axial ratio, volume‐to‐surface ratios, nuclear numerical density, profile area and cell volume. Serial sections were cut at 4 μm from routinely fixed, paraffin‐embedded specimens of each case and two sections, separated by 100 μm, were stained with haematoxylin and eosin. Random fields were photographed until at least 25 giant cell profiles had been recorded from each of the two sections of each lesion. The processed film was projected for point and intersect counting, the entire procedure ensuring double‐blind assessment of the cases under investigation. In general there was no significant difference for any of the parameters between the giant cells of central and peripheral jaw lesions. There was a significant difference, however, between the giant cells of central jaw lesions and long bone tumours in respect of both nuclear numerical density and mean absolute cell volume.